Associate Professor Yu-Fei Ao Associate Professor Yu-Fei Ao

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Associate Professor

 

Education and Research Experience

2003 - 2007   B.S.  College of Chemistry, Beijing Normal University, China

2008 - 2014   Ph.D.  Institute of Chemistry, Chinese Academy of Sciences (CAS), China (Supervisor: Prof. Mei-Xiang Wang)

2014 - 2019   Assistant Professor, Institute of Chemistry, Chinese Academy of Sciences (CAS), China

2019 - now    Associate Professor, Institute of Chemistry, Chinese Academy of Sciences (CAS), China

2021 - 2023   Visiting Scientist, Greifswald University, Germany. (Prof. Uwe T. Bornscheuer's Group)

 

Research Interests

Biocatalysis

 

Contact

CAS Key Laboratory of Molecular Recognition and Function

Institute of Chemistry, Chinese Academy of Sciences

Email: aoyufe@iccas.ac.cn

Tel: 86-10-62565610

Fax: 86-10-62564723

Address: Zhongguancun North First Street 2,100190 Beijing, China

ORCID number: 0000-0002-9415-2181

 

Publications

Zi-Lin Li, Shuxin Pei, Ziying Chen, Teng-Yu Huang, Xu-Dong Wang, Lin Shen,* Xuebo Chen,* Qi-Qiang Wang, De-Xian Wang, Yu-Fei Ao,* Machine learning-assisted amidase-catalytic enantioselectivity prediction and rational design of variants for improving enantioselectivity, Nat. Commun. 2024, 15, 8778.
 
Chenghai Sun,* Gen Lu, Baoming Chen, Guangjun Li, Ya Wu, Yannik Brack, Dong Yi, Yu-Fei Ao, Shuke Wu, Ren Wei, Yuhui Sun, Guifa Zhai,* Uwe T. Bornscheuer,* Direct asymmetric synthesis of β-branched aromatic α-amino acids using engineered phenylalanine ammonia lyases, Nat. Commun. 2024, 15, 8264.
 
Marian J. Menke, Yu-Fei Ao,* Uwe T. Bornscheuer,* Practical Machine Learning-Assisted Design Protocol for Protein Engineering: Transaminase Engineering for the Conversion of Bulky Substrates, ACS Catal. 2024, 14, 6462-6469.
 
Yu-Fei Ao,* Mark Dӧrr, Marian J. Menke, Stefan Born, Egon Heuson, Uwe T. Bornscheuer,* Data-Driven Protein Engineering for Improving Catalytic Activity and Selectivity, ChemBioChem 2024, 25, e202300754. (invited concept review)
 
Yu-Fei Ao,* Shuxin Pei, Chao Xiang, Marian J. Menke, Lin Shen,* Chenghai Sun, Mark Dörr, Stefan Born, Matthias Höhne, Uwe T. Bornscheuer,* "Structure- and data-driven protein engineering of transaminases for improving activity and stereoselectivity", Angew. Chem. Int. Ed. 2023, 62, e202301660.
 
Chao Xiang,+ Yu-Fei Ao,+ Matthias Höhne, Uwe T. Bornscheuer,* "Shifting the pH optima of (R)-selective transaminases by protein engineering", Int. J. Mol. Sci. 2022, 23, 15347.
 
Yu-Fei Ao,* Hui-Juan Hu, Cheng-Xin Zhao, Peng Chen, Tingting Huang, Hui Chen,* Qi-Qiang Wang, De-Xian Wang, Mei-Xiang Wang, "Reversal and amplification of the enantioselectivity of biocatalytic desymmetrization toward meso heterocyclic dicarboxamides enabled by rational engineering of amidase", ACS Catal. 2021, 11, 6900-6907.
 
Peng-Wei Xu, Xiao-Yuan Cui, Chen Chen, Feng Zhou, Jin-Sheng Yu, Yu-Fei Ao,* Jian Zhou,* "Enantioselective synthesis of Cα‑ tetrasubstituted N‑hydroxyl-α-amino nitriles via cyanation of ketonitrones using Me2(CH2Cl)SiCN", Org. Lett. 2021, 23, 8471-8476.
 
Hui-Juan Hu, Qi-Qiang Wang, De-Xian Wang, Yu-Fei Ao,* "Modification of the enantioselectivity of biocatalytic meso-desymmetrization for synthesis of both enantiomers of cis-1,2-disubstituted cyclohexane by Amidase engineering", Adv. Synth. Catal. 2021, 363, 4538-4543.
 
Hui-Juan Hu, Qi-Qiang Wang, De-Xian Wang, Yu-Fei Ao,* "Enantioselective biocatalytic desymmetrization for synthesis of enantiopure cis-3,4-disubstituted pyrrolidines", Green Synth. Catal. 2021, 2, 324-327.
 
Hui-Juan Hu, Peng Chen, Yu-Fei Ao,* Qi-Qiang Wang, De-Xian Wang, Mei-Xiang Wang, "Highly Efficient Biocatalytic Desymmetrization of Meso Carbocyclic 1,3-Dicarboxamides: A Versatile Route for Enantiopure 1,3-Disubstituted Cyclohexanes and Cyclopentanes", Org. Chem. Front. 2019, 6, 808-812.
 
Yu-Fei Ao,* Li-Bin Zhang, Qi-Qiang Wang, De-Xian Wang, Mei-Xiang Wang, Biocatalytic desymmetrization of prochiral 3-aryl and 3-arylmethyl glutaramides: Different remote substituent effect on catalytic efficiency and enantioselectivity, Adv. Synth. Catal. 2018, 360, 4594-4603.
 
Yu-Fei Ao, De-Xian Wang, Liang Zhao, Mei-Xiang Wang*, "Synthesis of quaternary carbon-containing and functionalized enantiopure 3-carbamoylcyclo-pentanecarboxylic acids from biocatalytic enantioselective desymmetrization of meso cyclopentane-1,3-dicarboxamides", Chem. Asian J. 2015, 10, 938-947.
 
Yu-Fei Ao, De-Xian Wang, Liang Zhao, Mei-Xiang Wang*, "Biotransformations of racemic 2,3-allenenitriles in biphasic systems: Synthesis and transfor-mations of enantioenriched axially chiral 2,3-allenoic acids and their derivatives", J. Org. Chem. 2014, 79, 3103-3110.
 
Yu-Fei Ao, Dong-Hui Leng, De-Xian Wang, Liang Zhao, Mei-Xiang Wang*, "Efficient synthesis of highly enantiopure β-lactam derivatives from biocatalytic transformations of amides and nitriles", Tetrahedron 2014, 70, 4309-4316.