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Conjugated polymers are characterized by a delocalized electronic structure that exhibits efficient coupling between optoelectronic segments. Excitons can be efficiently transferred to lower electron/energy acceptor sites over long distances to super-quench the fluorescence of conjugated polyelectrolytes or amplify the signals of acceptors. The integration of conjugated polymers with biomedical research has already shown profound effects on the diagnosis for the early detection of diseases related biomarkers, cell imaging, drug/gene delivery and diseases treatment, which leads to a multi-disciplinary scientific field in a context of chemistry, polymer science, materials, biology and medicine with an integrated perspective into both basic research and application issues.